| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.227141 |
| Chromosome: | chromosome 1 |
| Location: | 4492315 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g030600 | MAPKKK4 | (1 of 57) 2.7.10.2 - Non-specific protein-tyrosine kinase / Cytoplasmic protein tyrosine kinase; Mitogen-Activated Protein Kinase Kinase Kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCCGGCGTTGGTCATCAAGGATGTCGGC |
| Internal bar code: | GGGTTCCCGTCGTCTTAACCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1220 |
| LEAP-Seq percent confirming: | 99.1943 |
| LEAP-Seq n confirming: | 27824 |
| LEAP-Seq n nonconfirming: | 226 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCGCCAACACCAATAACAT |
| Suggested primer 2: | CGTACTGAGGGACAGGTGGT |