| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.227141 |
| Chromosome: | chromosome 1 |
| Location: | 4492340 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g030600 | MAPKKK4 | (1 of 57) 2.7.10.2 - Non-specific protein-tyrosine kinase / Cytoplasmic protein tyrosine kinase; Mitogen-Activated Protein Kinase Kinase Kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTCTGGCGGCGCCGCACAAGCCGCCGCC |
| Internal bar code: | CGGGCCGAGGCCGGTTCTATTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 582 |
| LEAP-Seq percent confirming: | 92.0975 |
| LEAP-Seq n confirming: | 3741 |
| LEAP-Seq n nonconfirming: | 321 |
| LEAP-Seq n unique pos: | 83 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCGCCAACACCAATAACAT |
| Suggested primer 2: | CGTACTGAGGGACAGGTGGT |