| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.227157 |
| Chromosome: | chromosome 16 |
| Location: | 1105460 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g649800 | PPR3,CYCR6 | (1 of 1) PF02984//PF13041 - Cyclin, C-terminal domain (Cyclin_C) // PPR repeat family (PPR_2); PentatricoPeptide Repeat protein 3 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCCTCGCGGTGAGGGCTGCGTTGTCTGAG |
| Internal bar code: | GTGAAGCGGGGATTGCGTAAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 656 |
| LEAP-Seq percent confirming: | 99.2289 |
| LEAP-Seq n confirming: | 1673 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGTTAAGGGTGTTGCGAT |
| Suggested primer 2: | ATATCACATGGGCTGAAGGC |