Insertion junction: LMJ.RY0402.227160_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.227160
Chromosome:chromosome 9
Location:6592766
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre09.g408400 TPT12,TPT13 (1 of 13) PTHR13244//PTHR32523//PTHR32523:SF8 - ZINC FINGER MYND DOMAIN CONTAINING PROTEIN 10 // FAMILY NOT NAMED // PHYTOL KINASE 1, CHLOROPLASTIC; Triose phosphate transporter 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATCGACCCCGTGCGCGACTCTTCGTAACC
Internal bar code:CTTGGAGGCGGCGCGGGGGGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:356
LEAP-Seq percent confirming:99.6669
LEAP-Seq n confirming:1197
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:ATTGTTGCCCACACTCTTCC
Suggested primer 2:GGCAGTTGCATGGGTTAGTT