Insertion junction: LMJ.RY0402.227162_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.227162
Chromosome:chromosome 17
Location:3030038
Confidence (%):73
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGTTCTCGCCCACGGCCCCTGCCTAGGTT
Internal bar code:GCTGACCAATCTAGGGATTCAT

Confirmation - LEAP-Seq

LEAP-Seq distance:945
LEAP-Seq percent confirming:94.3631
LEAP-Seq n confirming:3030
LEAP-Seq n nonconfirming:181
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GTGGTATTGATAATGGGGCG
Suggested primer 2:AAAGAGCATTGTGTACGGGG