| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.227185 |
| Chromosome: | chromosome 7 |
| Location: | 254733 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g313900 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGGTCATCGGGAGCTGCGAGGCCGGAGC |
| Internal bar code: | ATGTGAGGGTCGGGGTAGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 544 |
| LEAP-Seq percent confirming: | 98.563 |
| LEAP-Seq n confirming: | 1166 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCGCACCTTTCTGTGGTTC |
| Suggested primer 2: | GGTCGTAGTCCTCCTCCTCC |