| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.227200 |
| Chromosome: | chromosome 17 |
| Location: | 5720456 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g739000 | (1 of 2) PF11360 - Protein of unknown function (DUF3110) (DUF3110) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTACGGCATCCAATCATCAGGGCAGGGC |
| Internal bar code: | TCACTACGATGTTAAGTGATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1297 |
| LEAP-Seq percent confirming: | 98.6967 |
| LEAP-Seq n confirming: | 12495 |
| LEAP-Seq n nonconfirming: | 165 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGGTGTGGATAAGGACGC |
| Suggested primer 2: | TGTGTGTATGCGTGGATGTG |