| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.227219 |
| Chromosome: | chromosome 6 |
| Location: | 4844882 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278550 | (1 of 3) PTHR12385//PTHR12385:SF44 - CTL TRANSPORTER // PLASMA-MEMBRANE CHOLINE TRANSPORTER FAMILY PROTEIN | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGGAGGTGCAAGTCCTGTTAGACTTCGT |
| Internal bar code: | GCATTCGGTCACGGGGACCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 735 |
| LEAP-Seq percent confirming: | 99.1341 |
| LEAP-Seq n confirming: | 4465 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTTTCATTTGCGTTTTGGG |
| Suggested primer 2: | CAACGACGTCGAAACTTGAA |