| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.227230 |
| Chromosome: | chromosome 3 |
| Location: | 4966397 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g179961 | POLE1,POLE1-1 | (1 of 1) K02324 - DNA polymerase epsilon subunit 1 (POLE1); DNA polymerase epsilon subunit 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGTGGGTTGATGGTGGTGGGAGTGGGTG |
| Internal bar code: | CCTTTTTGCGCTCGCTGGATTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 544 |
| LEAP-Seq percent confirming: | 99.6356 |
| LEAP-Seq n confirming: | 3828 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGATTCCAGCACAAAAGCA |
| Suggested primer 2: | TGTGTGTGTGTGTGTGAGGG |