| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.227252 |
| Chromosome: | chromosome 7 |
| Location: | 5430234 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g350451 | (1 of 2) IPR001680//IPR017986//IPR020472//IPR027417 - WD40 repeat // WD40-repeat-containing domain // G-protein beta WD-40 repeat // P-loop containing nucleoside triphosphate hydrolase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCTGGCCGCGTGGTGTATGTGACCCCGC |
| Internal bar code: | GGCCGATCCTACCTCATTGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 553 |
| LEAP-Seq percent confirming: | 90.7569 |
| LEAP-Seq n confirming: | 7266 |
| LEAP-Seq n nonconfirming: | 740 |
| LEAP-Seq n unique pos: | 78 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACGAGCGATCGATAGGAG |
| Suggested primer 2: | CCAGATAGCTGTAGCTGCCC |