| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.227259 |
| Chromosome: | chromosome 10 |
| Location: | 5234896 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g457150 | (1 of 1) PF14648 - FAM91 C-terminus (FAM91_C) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGACGGAGCGCCGGCGGGGAGGACAAGGA |
| Internal bar code: | TTCGGTCATTGGGGCGTAATTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1113 |
| LEAP-Seq percent confirming: | 99.3327 |
| LEAP-Seq n confirming: | 12355 |
| LEAP-Seq n nonconfirming: | 83 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCGAGGTTACAATCATGGC |
| Suggested primer 2: | ACAAACATCCAACCCTCAGC |