| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.227259 |
| Chromosome: | chromosome 10 |
| Location: | 5234902 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g457150 | (1 of 1) PF14648 - FAM91 C-terminus (FAM91_C) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCTCACCCGTGCCGTACTCCACGCACCC |
| Internal bar code: | GCGCAGGGAGTGGGAGGAGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 611 |
| LEAP-Seq percent confirming: | 99.1459 |
| LEAP-Seq n confirming: | 1509 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCGAGGTTACAATCATGGC |
| Suggested primer 2: | ACAAACATCCAACCCTCAGC |