| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.227296 |
| Chromosome: | chromosome 4 |
| Location: | 3957306 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g231418 | (1 of 726) IPR011009 - Protein kinase-like domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGCTGGCAAGGACGTGAGCCCCGACTGG |
| Internal bar code: | CGGCACTGATGATGTTGGGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 602 |
| LEAP-Seq percent confirming: | 99.1484 |
| LEAP-Seq n confirming: | 815 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGAGACAAGGAGTGGCTG |
| Suggested primer 2: | AGACCTTGCAAAGCCCCTAT |