| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.227325 |
| Chromosome: | chromosome 1 |
| Location: | 6474494 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g046052 | (1 of 6) 2.7.11.21 - Polo kinase / Polo-like kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCAGAGGGCCGCGTGAGGGAGTGGGGCG |
| Internal bar code: | GCGCGTTTCTGGGATGCCTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 436 |
| LEAP-Seq percent confirming: | 83.7353 |
| LEAP-Seq n confirming: | 9375 |
| LEAP-Seq n nonconfirming: | 1821 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGCATGGAGGTAGCAGTA |
| Suggested primer 2: | TCCAACACCAGCACTAGCAG |