| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.227338 |
| Chromosome: | chromosome 7 |
| Location: | 1814171 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325727 | (1 of 3) PTHR10566:SF76 - PROTEIN Y32H12A.7 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGTGTGTGTGTGTGTGTGTATGTTCCAC |
| Internal bar code: | TGTGTAAGCGGATGCTGCAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 582 |
| LEAP-Seq percent confirming: | 90.0765 |
| LEAP-Seq n confirming: | 4947 |
| LEAP-Seq n nonconfirming: | 545 |
| LEAP-Seq n unique pos: | 97 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACAACACTGCACATGGGG |
| Suggested primer 2: | TGTGTTGATGCATGTGTTGG |