| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.227353 |
| Chromosome: | chromosome 12 |
| Location: | 1187435 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g489850 | STPK10,STK10 | Serine/threonine protein kinase; (1 of 107) PF00069//PF07714 - Protein kinase domain (Pkinase) // Protein tyrosine kinase (Pkinase_Tyr) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGACGGTATTAATGGCAGGAATGCCTTCG |
| Internal bar code: | GCTAGATGCGCGGGAGTTACCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 668 |
| LEAP-Seq percent confirming: | 99.0953 |
| LEAP-Seq n confirming: | 6572 |
| LEAP-Seq n nonconfirming: | 60 |
| LEAP-Seq n unique pos: | 114 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGAACCTGCAGGATGAGT |
| Suggested primer 2: | GCTTCGACCCCACATGTACT |