| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.227358 |
| Chromosome: | chromosome 17 |
| Location: | 3185321 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g721950 | (1 of 1) PTHR11685:SF119 - E3 UBIQUITIN-PROTEIN LIGASE ARI2-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTACGCGCACACGCGCGTCCGAGTCGATGG |
| Internal bar code: | GCGCGTTATAATAGAATCCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 764 |
| LEAP-Seq percent confirming: | 96.1004 |
| LEAP-Seq n confirming: | 2489 |
| LEAP-Seq n nonconfirming: | 101 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCTCCACCTTGAACTTGC |
| Suggested primer 2: | GACAGCTACAGCAAGGAGGG |