| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.227377 |
| Chromosome: | chromosome 16 |
| Location: | 3873135 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g690050 | HCS2 | Cytochrome c heme lyase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACTTATGTAGCCCTTACGTGTGACACTAC |
| Internal bar code: | GGTCTTTCCTTGGTAGAACAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 453 |
| LEAP-Seq percent confirming: | 94.2333 |
| LEAP-Seq n confirming: | 25394 |
| LEAP-Seq n nonconfirming: | 1554 |
| LEAP-Seq n unique pos: | 153 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACTGGACTGGTGGGAACTG |
| Suggested primer 2: | GTTGAGGGACTGTTGAGGGA |