| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.227383 |
| Chromosome: | chromosome 7 |
| Location: | 2591786 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g330000 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCGCCAAGAGGGATATTGACGCGCTGCA |
| Internal bar code: | AGTTCCTTCCTTCATGCGGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1088 |
| LEAP-Seq percent confirming: | 98.9295 |
| LEAP-Seq n confirming: | 32067 |
| LEAP-Seq n nonconfirming: | 347 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAAACTCGTCACACCCAC |
| Suggested primer 2: | CCTAGCTCTCCCTGAACACG |