| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.227387 |
| Chromosome: | chromosome 10 |
| Location: | 2749081 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g438600 | (1 of 1) PF09072 - Translation machinery associated TMA7 (TMA7) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTGGCACGCGGCACGTGGACTACCCTGC |
| Internal bar code: | GCTTGCTTACGTACGGGTGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 539 |
| LEAP-Seq percent confirming: | 99.2312 |
| LEAP-Seq n confirming: | 3356 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCACCGTACTACCTCCACG |
| Suggested primer 2: | TCTAGGAAACGGCCAAAATG |