Insertion junction: LMJ.RY0402.227436_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.227436
Chromosome:chromosome 6
Location:3543350
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g278111 (1 of 6) IPR000595//IPR005821//IPR018490 - Cyclic nucleotide-binding domain // Ion transport domain // Cyclic nucleotide-binding-like CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTTCTTGAAGATCCACAGGATGCGGAAGA
Internal bar code:CACGGCGCCATAGTACCCAAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:464
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:418
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:AGCAGGGTTACAGGAGGGTT
Suggested primer 2:AGGGGAGGTTTCTGGAAAGA