| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.227464 |
| Chromosome: | chromosome 13 |
| Location: | 618471 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g565750 | EFG4 | (1 of 1) K14416 - elongation factor 1 alpha-like protein (HBS1); Translation elongation factor Tu family protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGAGGGCGTGCGCACCGTACTCAAAGCG |
| Internal bar code: | AAGAATCGCGGCACCTTGCGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 196 |
| LEAP-Seq percent confirming: | 96.25 |
| LEAP-Seq n confirming: | 385 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATGGCTAGCTAGAATCCG |
| Suggested primer 2: | CCTGCAGACGACAAACAAAA |