| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.227472 |
| Chromosome: | chromosome_14 |
| Location: | 912006 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre14.g613600 | HSP70H | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGGCCAATGACGGCATTGCGGCTGGCCACT |
| Internal bar code: | ATCAATTTTTATGCGTAAATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 413 |
| LEAP-Seq percent confirming: | 99.4514 |
| LEAP-Seq n confirming: | 10514 |
| LEAP-Seq n nonconfirming: | 58 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACTGCTGCCACTGTTCCTG |
| Suggested primer 2: | AAGAGTGTGTGGATGAGGGG |