Insertion junction: LMJ.RY0402.227474_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.227474
Chromosome:chromosome 10
Location:2784200
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g439000 (1 of 3) 3.6.3.47 - Fatty-acyl-CoA-transporting ATPase; Putative ABC transporter intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TATCACACAACAAAGGAGCCCGCACCTGGA
Internal bar code:GGGCGAGCGTGACTGGCGTCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:762
LEAP-Seq percent confirming:97.8277
LEAP-Seq n confirming:5314
LEAP-Seq n nonconfirming:118
LEAP-Seq n unique pos:88

Suggested primers for confirmation by PCR

Suggested primer 1:CGGTTTGCACATACATCAGG
Suggested primer 2:CGTACTGGCAGGAGGAGAAG