| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.227476 |
| Chromosome: | chromosome 4 |
| Location: | 2690400 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g223250 | LCIE,LCIE1 | Low-CO2 inducible protein E | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTTCGAGCCCACTGCTGCCCAGCTATGGA |
| Internal bar code: | TTACAAAAGTGTGGTCCCATGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1060 |
| LEAP-Seq percent confirming: | 99.3152 |
| LEAP-Seq n confirming: | 19143 |
| LEAP-Seq n nonconfirming: | 132 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATGGAGAGAAGCAAGCGT |
| Suggested primer 2: | TGACAAACACAGCAATGGGT |