| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.227480 |
| Chromosome: | chromosome 7 |
| Location: | 6399582 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g357900 | (1 of 1) K08054 - calnexin (CANX) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCGGTGGGTGGGCAGATGAGAAGGGCCA |
| Internal bar code: | TAATGGGGCGACGGCTCATCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 608 |
| LEAP-Seq percent confirming: | 97.6755 |
| LEAP-Seq n confirming: | 2101 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACACTCGTCAATAACGCCC |
| Suggested primer 2: | CGGAAGGTGAGAAAGAGGTG |