| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.227483 |
| Chromosome: | chromosome 13 |
| Location: | 3625553 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g588550 | SYP1 | Plasma membrane Qa-SNARE, Syx1/Sso1/Syntaxin1/Syp1 family (Qa.IV); (1 of 1) K08486 - syntaxin 1B/2/3 (STX1B_2_3) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCCTGCCAGGGGAACCCCTGTCTCCCGC |
| Internal bar code: | GTCACCGGTGGCACGCCAACGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 464 |
| LEAP-Seq percent confirming: | 93.4097 |
| LEAP-Seq n confirming: | 326 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGCTGACACTGATGCTGT |
| Suggested primer 2: | GATTGCCGTCAACACAAATG |