Insertion junction: LMJ.RY0402.227496_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.227496
Chromosome:chromosome 9
Location:6087093
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g403330 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAAACCGCGCAAACGACACCGGCACACACA
Internal bar code:GTGGGGAGTCCATATCGGCGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:401
LEAP-Seq percent confirming:96.8011
LEAP-Seq n confirming:696
LEAP-Seq n nonconfirming:23
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:CGGATGAGCTGTGTAGACGA
Suggested primer 2:AACAATGCCTCGGAAACAAC