| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.227503 |
| Chromosome: | chromosome 12 |
| Location: | 1101389 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g490650 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCGAGACCCCAGGTTCGTAGCTGCATCGC |
| Internal bar code: | AGGCACCATGAACTGACGAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 590 |
| LEAP-Seq percent confirming: | 98.6294 |
| LEAP-Seq n confirming: | 3598 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACTGACTGTTGGGGTCTGG |
| Suggested primer 2: | GCTAGTTCCATTTTGCTCGC |