| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.227522 |
| Chromosome: | chromosome 6 |
| Location: | 4860747 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278700 | Conserved Ankyrin-Repeat Protein; (1 of 1) PTHR24193:SF69 - IQ MOTIF AND ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN LOC642574 HOMOLOG-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGGACACGTGCGTTGGTAAGCAAGAAGCT |
| Internal bar code: | CTATGGAGGGACTCATGATTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 112 |
| LEAP-Seq percent confirming: | 99.1304 |
| LEAP-Seq n confirming: | 114 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTTGCCTCTCTCATGTGC |
| Suggested primer 2: | ACTCGTCACTCCCTTCATGG |