Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.227547 |
Chromosome: | chromosome_12 |
Location: | 9107516 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g542700 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CTTTGGTTGAAGCTGGCATGCCCGCGGCAA |
Internal bar code: | GGTACGCTCGGAGGTCCGTTTT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 802 |
LEAP-Seq percent confirming: | 99.5169 |
LEAP-Seq n confirming: | 4326 |
LEAP-Seq n nonconfirming: | 21 |
LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GATGGATACGGCAGTAGCGT |
Suggested primer 2: | CTCGATGGAAGCAGTTGTCA |