Insertion junction: LMJ.RY0402.227548_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.227548
Chromosome:chromosome_7
Location:5180769
Confidence (%):58
Locus systematic id Locus common name Defline Orientation Feature
Cre07.g348450 antisense 5'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):CGTCGGCGGCACGCCCTTTGCGTGCCTTTA
Internal bar code:ATGTGACCGGAGGGTCGATTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:583
LEAP-Seq percent confirming:80.0578
LEAP-Seq n confirming:277
LEAP-Seq n nonconfirming:69
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:GTAGGCAATTCGGAACGTGT
Suggested primer 2:CCGAACAGTCGGAAGCTAAG