| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.227599 |
| Chromosome: | chromosome 4 |
| Location: | 2312010 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g220576 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGGATGACGCCCGTCTCTGTCTCCTTCA |
| Internal bar code: | GCGGACAGCAGGGATCAAGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 481 |
| LEAP-Seq percent confirming: | 95.7904 |
| LEAP-Seq n confirming: | 1115 |
| LEAP-Seq n nonconfirming: | 49 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGTGTCCCAACTCACCTT |
| Suggested primer 2: | CCCAACTCCTCACACCAACT |