Insertion junction: LMJ.RY0402.227616_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.227616
Chromosome:chromosome 11
Location:497691
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre11.g467597 (1 of 1) IPR000104//IPR003439//IPR003593//IPR013525//IPR013581//IPR027417 - Antifreeze protein, type I // ABC transporter-like // AAA+ ATPase domain // ABC-2 type transporter // Plant PDR ABC transporter associated // P-loop containing nucleoside triphosphate hydrolase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCTGCCGCTGCTGCTGCTGCCGCTGCCGC
Internal bar code:CCATGGCTTAACCTCGCACCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:315
LEAP-Seq percent confirming:90.8607
LEAP-Seq n confirming:4096
LEAP-Seq n nonconfirming:412
LEAP-Seq n unique pos:36

Suggested primers for confirmation by PCR

Suggested primer 1:CCACATCCTCTCCCTCACAT
Suggested primer 2:TCCTCCTCGCTCTACTGCAT