| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.227645 |
| Chromosome: | chromosome 10 |
| Location: | 6426921 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g465850 | (1 of 1) K12572 - PAB-dependent poly(A)-specific ribonuclease subunit 3 (PAN3) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACGTTCCTCATGCACCCTCCGCTTTTCAA |
| Internal bar code: | GTTAGTCCCGCATTCCTTACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 698 |
| LEAP-Seq percent confirming: | 99.8049 |
| LEAP-Seq n confirming: | 7675 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACCAGATTGAAGTCACGG |
| Suggested primer 2: | CTCGTGACGCCTACCTGTG |