Insertion junction: LMJ.RY0402.227673_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.227673
Chromosome:chromosome 1
Location:7523474
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g054150 NTRC1,NTR4 (1 of 1) PTHR10438//PTHR22912//PTHR22912:SF48 - THIOREDOXIN // DISULFIDE OXIDOREDUCTASE // NADPH-DEPENDENT THIOREDOXIN REDUCTASE 3; NADPH-dependent thioredoxin reductase C, chloroplastic 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGAATGCCTGTGACTGCGCGTGTGCGTGT
Internal bar code:TGTGAGCGACGACGGCGGTGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:985
LEAP-Seq percent confirming:98.7142
LEAP-Seq n confirming:11516
LEAP-Seq n nonconfirming:150
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:CGTTGTTGCAGCAGATCAGT
Suggested primer 2:GGCTGCCTACTGAGGAACAC