| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.227679 |
| Chromosome: | chromosome 8 |
| Location: | 956853 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g361950 | FAL10,FAP153 | Flagellar Associated Membrane Protein 153 with PAS Sensory Domain; (1 of 28) PTHR31600//PTHR31600:SF2 - FAMILY NOT NAMED // TINY MACROCYSTS PROTEIN B-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCGATTGGCTTAGCGGAATGAAGCGCTC |
| Internal bar code: | AATTGGTGCACAGGATGGGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 614 |
| LEAP-Seq percent confirming: | 97.2323 |
| LEAP-Seq n confirming: | 1616 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCCCAGTGTGTATGTAACG |
| Suggested primer 2: | TACGTTACAGTCACTCGCGG |