Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.227706 |
Chromosome: | chromosome 7 |
Location: | 1566640 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre07.g324500 | MMP22 | Matrix metalloproteinase; (1 of 40) 3.4.24.38 - Gametolysin / Lysin | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCGCCATGCCTTACGCAGCGGGTGGGCC |
Internal bar code: | GCGGGGACATGGGCTCGGATCA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 512 |
LEAP-Seq percent confirming: | 95.6734 |
LEAP-Seq n confirming: | 4113 |
LEAP-Seq n nonconfirming: | 186 |
LEAP-Seq n unique pos: | 61 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AAGCACGAAAATCACCCAAC |
Suggested primer 2: | GACAGCAATGTGGTGGTCAG |