| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.227706 |
| Chromosome: | chromosome 7 |
| Location: | 1566648 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g324500 | MMP22 | Matrix metalloproteinase; (1 of 40) 3.4.24.38 - Gametolysin / Lysin | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCACATTGCACTGCATTATAATTGTTTTT |
| Internal bar code: | CAGTTGGGCAATTATTCTACTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1026 |
| LEAP-Seq percent confirming: | 99.2449 |
| LEAP-Seq n confirming: | 10252 |
| LEAP-Seq n nonconfirming: | 78 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCACGAAAATCACCCAAC |
| Suggested primer 2: | GACAGCAATGTGGTGGTCAG |