Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.227718 |
Chromosome: | chromosome_14 |
Location: | 806892 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre14.g613100 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GCATGTACGGTGTCTGCGTCTAGTTGCGCT |
Internal bar code: | GATCCCGCATACATCGGGACAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 650 |
LEAP-Seq percent confirming: | 99.1928 |
LEAP-Seq n confirming: | 3195 |
LEAP-Seq n nonconfirming: | 26 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGAAGAAGCTAAGGGCGGTC |
Suggested primer 2: | GCGATTTCGGTGGTGTAAGT |