| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.227738 |
| Chromosome: | chromosome 2 |
| Location: | 3201646 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095071 | (1 of 2) K01148 - poly(A)-specific ribonuclease (PARN) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGATTGCTGAGTTGAGGAAGTATTGTAGC |
| Internal bar code: | AGGCTAGAAGATGATATTCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1141 |
| LEAP-Seq percent confirming: | 98.709 |
| LEAP-Seq n confirming: | 20950 |
| LEAP-Seq n nonconfirming: | 274 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCATGTAGCCCAGGTAAC |
| Suggested primer 2: | ACAACCTAACCCGTAACCCC |