Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.227741 |
Chromosome: | chromosome_6 |
Location: | 7002733 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre06.g296700 | HYDG1,HYDG | Hydrogenase assembly factor/biotin synthase | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ATTCATTGTTAAATGGCCATGCACAGGTGC |
Internal bar code: | GCTTGGTAGCGCAAGGTGGTGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 638 |
LEAP-Seq percent confirming: | 97.947 |
LEAP-Seq n confirming: | 2624 |
LEAP-Seq n nonconfirming: | 55 |
LEAP-Seq n unique pos: | 57 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GACCAACTCTTTCAGCCAGC |
Suggested primer 2: | ACTAGCCAACCCCGAAGTTT |