| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.227759 |
| Chromosome: | chromosome 13 |
| Location: | 1541062 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g572900 | THO3 | (1 of 1) 3.4.24.70 - Oligopeptidase A; Oligoendopeptidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTGGTTTAGTTCGTGCTGGTATTTACAA |
| Internal bar code: | GGTTTACCGGCGCACTAACGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 85 |
| LEAP-Seq percent confirming: | 27.7778 |
| LEAP-Seq n confirming: | 65 |
| LEAP-Seq n nonconfirming: | 169 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGAGCTGGAGAGAGGGAGG |
| Suggested primer 2: | TACCAGCCCAAACTAAACCG |