| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.227759 |
| Chromosome: | chromosome 13 |
| Location: | 1541064 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g572900 | THO3 | (1 of 1) 3.4.24.70 - Oligopeptidase A; Oligoendopeptidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCAAGGCTAGCTGGCGGAGCACAGGGCAG |
| Internal bar code: | TAGACGAGCTAATTCAAAGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 361 |
| LEAP-Seq percent confirming: | 81.0101 |
| LEAP-Seq n confirming: | 802 |
| LEAP-Seq n nonconfirming: | 188 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCAGCCCAAACTAAACCG |
| Suggested primer 2: | AAGAGCTGGAGAGAGGGAGG |