| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.227771 |
| Chromosome: | chromosome_13 |
| Location: | 3395919 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre13.g587050 | ERF1 | Eukaryotic release factor | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GAGGGGGCGATGGAGGAGGGGGCGGCTGCG |
| Internal bar code: | TTAGGCCATGCGGAGGAAATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 779 |
| LEAP-Seq percent confirming: | 88.6096 |
| LEAP-Seq n confirming: | 13715 |
| LEAP-Seq n nonconfirming: | 1763 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCAGTGTGACGGTACTTT |
| Suggested primer 2: | TACCTCTCGCACGCCTAGAT |