| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.227775 |
| Chromosome: | chromosome 12 |
| Location: | 4023022 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g517100 | (1 of 2) 5.5.1.6 - Chalcone isomerase / Chalcone--flavanone isomerase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGATGGGATGCCAGGCGAGCATTGCCACT |
| Internal bar code: | CTTGACCATGCGAGGCTGGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 784 |
| LEAP-Seq percent confirming: | 99.3011 |
| LEAP-Seq n confirming: | 12787 |
| LEAP-Seq n nonconfirming: | 90 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGGCATACATTTCACGTC |
| Suggested primer 2: | TTGGAGTTTGAGCTGCAATG |