Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.227790 |
Chromosome: | chromosome_3 |
Location: | 2388279 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g158850 | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CTCAACCATGGGGTGCTCACTGTTGGGCAC |
Internal bar code: | TCACTGCCGAGACAGTGGGGGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 618 |
LEAP-Seq percent confirming: | 98.3972 |
LEAP-Seq n confirming: | 3929 |
LEAP-Seq n nonconfirming: | 64 |
LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGCTGAGAGTCAACAATGGC |
Suggested primer 2: | GATGAAGTCCAGGCAGAAGC |