Insertion junction: LMJ.RY0402.227790_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.227790
Chromosome:chromosome 3
Location:2388279
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g158850 (1 of 1) PTHR24413:SF79 - BTB/POZ DOMAIN-CONTAINING PROTEIN 9 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCAATCCAGAACTTGCTGAACACCTTGCC
Internal bar code:CATTCGATCAATCGGATCGGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:948
LEAP-Seq percent confirming:99.5371
LEAP-Seq n confirming:12902
LEAP-Seq n nonconfirming:60
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:TGCTGAGAGTCAACAATGGC
Suggested primer 2:GATGAAGTCCAGGCAGAAGC