| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.227790 |
| Chromosome: | chromosome_10 |
| Location: | 6511068 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g466650 | FAP252 | Flagellar Associated Protein | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTCTTTTTGTAGGTCTCCTGTAAACCCTGG |
| Internal bar code: | AGATGGCGCATGTTTTATATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 809 |
| LEAP-Seq percent confirming: | 98.4064 |
| LEAP-Seq n confirming: | 494 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTACCGTATGGTGCAGTT |
| Suggested primer 2: | GTCGCCCATCTATCCTTCAA |