| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.227791 |
| Chromosome: | chromosome 7 |
| Location: | 4092534 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g340650 | CGL149 | Conserved in the Green Lineage; (1 of 1) PF09430 - Protein of unknown function (DUF2012) (DUF2012) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTTTACCGACTGTCCTACGGGTGCGACCG |
| Internal bar code: | GAGGAGAGTAAGTAGGGGCGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 729 |
| LEAP-Seq percent confirming: | 98.062 |
| LEAP-Seq n confirming: | 1012 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATCGTGTTCTCGCTGTTC |
| Suggested primer 2: | AACTTTCAATGCATACCGGC |